• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    METHODS TO PRODUCE A PHYTASE VARIANT, TO IMPROVE THE NUTRITIONAL VALUE OF AN ANIMAL FEED, FOR THE TREATMENT OF VEGETABLE PROTEINS AND TO PRODUCE A FERMENTATION PRODUCT, PHYTASE VARIANT, SEQUENCE OF ISOLATED NUCLEIC ACIDES, ISOLATED NUCLEIC ACID, CONNECTED NUCLEIC ACID, NUCLEIC ACID, NUCLEIC ACID. RECOMBINANT, RECOMBINANT HOST CELL, TRANSGENIC PLANT, COMPOSITION, PROCESS TO REDUCE FITANE LEVELS IN ANIMAL STERI, AND, USE OF PHYTASE The present invention relates to a method for producing variants of phytase that exhibit at least 74% identity with one phytase derived from Citrobacter braakii, and comprise at least two additional disulfide bonds compared to this phytase. These phytase variants have modified, preferably improved, properties such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, less sensitivity to protease and / or a modified glycosylation pattern. The invention also concerns the Variants produced, DNA encoding these phytases, methods of their production, as well as their use, for example, in animal feed and animal feed additives.
    公开号:BR112012023840A2
    申请号:R112012023840-0
    申请日:2011-03-25
    公开日:2020-07-28
    发明作者:Lars Kobberoee Skov；Leonardo De Maria；Tomoko Matsui
    申请人:Novozymes A/S；
    IPC主号:
    专利说明:

    “METHODS TO PRODUCE A PHYTASE VARIANT, TO
    IMPROVE THE NUTRITIONAL VALUE OF AN ANIMAL FEED AND TO PRODUCE A FERMENTATION PRODUCT, VARIABLE OF PHYTASE, ISOLATED NUCLEIC ACID SEQUENCE, 5 NUCLEIC ACID CONSTRUCT, RECOMBINANT EXPRESSION VECTOR, HOMEROMINATION, RECOMMENDED CELL, RECOMMENDED CELL, RECOMMENDED USE, COMPOSITION."
    SEQUENCE LIST REFERENCE This order contains a sequence listing in computer readable form. The computer-readable form is incorporated by reference here.
    FIELD OF THE INVENTION The present invention relates to a method of producing variants of phytase which exhibit at least 74% identity with a phytase derived from Citrobacter braakii ATCC 51113, and comprise the establishment of at least two disulfide bridges that are not among the four naturally occurring disulfide bridges compared to these and closely related phytases (that is, it is a variant of this). The invention also concerns DNA encoding these phytases, the variants produced, as well as their use, for example, in animal feed and animal feed additives. The mature phytase part of Citrobacter braakii ATCC 51113 is included in the sequence listing as SEQ ID NO: 2.
    BACKGROUND OF THE INVENTION Basics of the technique Phytases are well known enzymes, since it is advantageous to add them to food products for animals, including humans. Phytases have been isolated from several sources, including
    权利要求:
    Claims (25)
    [1]
    1. Method for producing a variant of phytase, characterized by the fact that it has at least 74% identity with SEQ ID NO: 2, and comprises the establishment of at least two disulfide bridges 5 compared to SEQ ID NO: 2, in which the said two disulfide bridges are not among those four naturally occurring in positions 77/108, 133/407, 178/187 and 381/390 with the numbering provided in SEQ ID NO: 2.
    [2]
    2. Method according to claim 1, characterized by the fact that the at least two disulfide bridges are established in the positions selected from the group consisting of the position pairs: A) 52/99; B) 31/177; C) 46/91; D) 141/199; E) 31/176; F) 59/100; and G) 162/247.
    [3]
    3. Method according to claims 1-2, characterized by the fact that the number of disulfide bridges established is two, using the following combinations of position pairs: A + B, A + C, A + D, A + E , A + F, A + G, B + C, B + D, B + E, B + F, B + G, C + D, C + E, C + F, C + G, D + E, D + F, D + G, E + F, E + G, and F + G, where A means 52/99; B means 31/177; C means 46/91; D means 141/199; E means 31/176, F means 59/100; and G means 162/247.
    [4]
    4. Method according to claims 1-2, characterized by the fact that the number of disulfide points established is three, using the following combinations of position pairs: A + B + C, A + B + D, A + B + E, A + B + F, A + B + G, A + C + D, A + C + E, A + C + F, A + C + G, A + D + E, A + D + F , A + D + G, A + E + F, A + E + G, A + F + G, B + C + D, B + C + E, B + C + F, B + C + G, B + D + E, B + D + F, B + D + G, B + E + F, B + E + G, B + F + G, C + D + E, C + D + F, C + D + G, C + E + F, C + E + G, C + F + G, D + E + F, D + E + G, D + F + G, and E + F + G, where A means 52/99; B means 31/177; C means 46/91; D means 141/199; E means 31/176, F means 59/100; and G means 162/247.
    [5]
    5. Method according to claims 1-2, characterized by the fact that the number of disulfide bridges established is four, using the following combinations of position pairs: A + B + C + D, A + B + C + E , A + B + C + F, A + B + C + G, A + B + D + E, A + B + D + F, A + B + D + G, A + B + E + F, A + B + E + G, A + B + F + G, A + C + D + E, 5 A + C + D + F, A + C + D + G, A + C + E + F, A + C + E + G, A + C + E + H, A + C + F + G, A + D + E + F, A + D + E + G, A + D + F + G, A + E + F + G, B + C + D + E, B + C + D + F, B + C + D + G, B + C + E + F, B + C + E + G, B + C + F + G, B + D + E + F, B + D + E + G, B + D + F + G, B + E + F + G, C + D + E + F, C + D + E + G, C + D + F + G, C + E + F + G, C + E + F + H, and D + E + F + G, where A means 52/99; B means 31/177; C means 46/91; D means 141/199; E means 31/176, F means 59/100; and G means 162/247.
    [6]
    Method according to claims 1 to 5, characterized in that it additionally comprises a modification in at least one position selected from the following: 1, 2, 3, 4, 5, 6, 24, 31, 35, 41 , 45, 46, 52, 53, 55, 56, 57, 59, 60, 64, 66, 73, 74, 75, 76, 79, 80, 81, 82, 83, 84, 87, 90, 91, 94 , 100, 104, 105, 106, 107, 109, 111, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 126, 129, 130, 132, 134, 136, 137 , 138, 139, 142, 146, 148, 154, 155, 157, 161, 162, 164, 167, 171, 173, 175, 176, 177, 179, 180, 181, 182, 183, 184, 185, 186 , 188, 190, 193, 196, 200, 202, 203, 205, 207, 211, 213, 215, 217, 218, 223, 231, 239, 240, 241, 247, 252, 254, 257, 266, 273 , 276, 279, 280, 281, 282, 283, 284, 285, 286, 289, 294, 299, 300, 308, 314, 316, 321, 324, 330, 331, 336, 338, 339, 340, 344 , 345, 348, 351, 355, 361, 362, 363, 364, 369, 371, 372, 373, 375, 379, 385, 386, 392, 406, 409, 410, and 411.
    [7]
    Method according to claim 6, characterized in that it comprises at least one of the following modifications: 1 *, 1H, K, R, Q, 2D, 2 *, 3 *, 4P, 5P, 6L, 24R, E , 31T, K, N, 35Y, 41P, 45R, 46D, E, 52E, R, 53V, Q, K, 53V, 55D, I, 56T, 57Y, V, 60P, H, K, R, 66Y, 73D , P, 74P, S, Y, A, 75T, 76G, P, 79F, 80L, 81E, 82E, 83I, 84Y, 87T, 90V, Y, 91P,
    100Y, 104I, A, 105F, E, 106A, G, 107D, E, G, 109A, G, 111P, 114H, N, T, 115Q, 116A, E, P, T, Q, 117D, E, K, 118V, I, L, M, T, 119G, K, R, S, 120K, S, T, Q, R 121A, D, M, P, T, V 121I, 122D, 123P, S, 124L, T, V, N126D, K129G 129I, R, P, Q, 130K, V, 132I, 134T 136P, 137P, 138F, 139N, 142D, 146D, 148T 5 154P, Q, V 155F, 157F, 161P, 164D, E, 167Q , 171T, 173P, T 175L, 177I, 179G, I, K, N, Q, 180A, E, G, T, 181D, G, I, K, L, S, W 182G, N, R, V, H , K, S, Q, 183RA, L, P, S, V, Q, 184RT, 184 *, 185A, S, 185 *, 186 *, 188P, 193F, 196Q, 200K, R, 202N, H, 203D, E, T, L, 205P, 207G, S, 211C, 213A, 215M, 218Q, 223E, 231P, 239Q, 240P, 241Q, 252H, L, 254Y, 257E, 266M, 273L, Q, 276K, R, 279S, 280P, 281H, 282P, 283P, 284P, 285G, N, R, E285Y, 286D, T, K, Q, 289P, 294T, 299L, 300V, 308A, 314G, N, 316D, 321A, 324N, 330D, 331K, 336R, 338N, 339D, 340A, I, 343D, 344S, 345N, 348H, 351Y, 355P, 361V, 362K, R, I362F, I362L, 362M, 363R, 364Q, 369N, 371P, 373T, 375N, 379K, R, 385D, 386I, 392I, 406A, 409D, E, 410D, E, and / or 411R, K.
    [8]
    Method according to any one of claims 1 to 7, characterized in that the amino acids at position 179, 180, 181, 182, 183, 184, 185 and 186 have been replaced by QADKP, GEDKP, NGISA, IAGKS, KEKHQ , KEKQQ, KEKKV, or KTDKL ..
    [9]
    Method according to any one of claims 1 to 8, characterized in that it comprises a combination of modifications selected from the following: 1 * / 2 *, 1 * / 2 * / 3 *, 55D / 331K, 111P / 241Q , 179K / 180E / 181K / 182H / 183Q / 184 * / 185 * / 186 *, 179K / 180E / 181K / 182Q / 183Q / 184 * / 185 * / 186 *, 179K / 180E / 181K / 182K / 183V / 184 * / 185 * / 186 *, 179K / 180T / 181D / 182K / 183L / 184 * / 185 * / 186 *, 114T / 115Q / 116A / 117D / 118T / 119S / 120S / 121P / 122D / 123P / 124L, and 114T / 115Q / 116T / 117D / 118T / 119S / 120S / 121P / 122D / 123P / 124L.
    [10]
    Method according to any one of claims 1 to 9, characterized in that the substitutions for establishing the disulfide bridges are: A ': G52C / A99C, B': N31C / T177C, C ': W46C / Q91C, 5 D ': K141C / V199C, E': N31C / E176C, F ': G59C / F100C, and / or G': S162C / S247C.
    [11]
    11. Method according to claim 10, characterized in that it comprises an additional modification selected from the following: E1K, E1Q, E2D, M6L, K24E, K24R, D31K, D31N, D35Y, G45R, G52R, E53K, E53V, V55I , S56T, E57V, Q60H, Q60K, Q60R, F66Y, N73D, N73P, N74P, N74S, N74Y, Q75T, T76G, T76P, S79F, P80L, G81E, V83I, A87T, D90V, D90Y, F100Y, L104I, Y114 , K120R, T121I, T121P, N126D, K129G, M130K, M130V, T132I, S134T, L138F, K139N, N142D, E146D, A148T, L154Q, L154V, Y155F, S173T, T18I18, 18, 18, 18, 18, 18, 18, 18 , E182H, E182I, E182N, E182R, E182V, K183P, K183R, S184R, S184T, T185A, T185S, L193F, D202H, N203D, N203E, P207S, S211C, S213A, E26, E25, Q252, Q252, , N286T, A300V, G321A, K336R, K338N, T340A, T340I, N343D, T344S, D345N, Q348H, D361V, I362F, I362L, I362M, Q363R, P364Q, K369N, A371P, K37T modifications selected from the group consisting of: K24E / Q60H; Q60K / V83I; N126D / T340I; K24E / L392I; F66Y / Y114N; T132I / P364Q; N73P / N74S; N73P / N74P; Q75T / T76G; D202H / N203E; Y155F / T177I; Q252L / Q348H; K24E / N74Y; N126D / L279S; E2D / R266M; E118V / A300V; K24E / S173T; E53K / N343D; T76P / S213A; L138F / L193F; Q252L / K373T; G45R / Q257E / N286T; K139N / P207S / R266M; D31N / D35Y / M130V; F100Y / S134T / P207S; T177I / S184R / T185S; E1K / K24E / Q60H;
    K24E / Q60H / Q363R; T181W / E182N / K183R; T181W / E182H / K183R; T181W / E182R / K183R; T181W / E182A / K183R; A0T / K129G / N286D; E1K / E53K / F66Y; M130K / N203D / L279S; S56T / Q252L / S386I; L154Q / T177I / T344S; K24E / V55I / T181S; K24R / T177I / D345N; 5 G52R / E182G / R266M, A0T / T177I / S184T / T185A, Q60R / F66Y / L104I / I362L, K24E / Q60H / E146D / Q252L, Q60K / T132I / Q257E / N284T, Q60R / D90V25 / N73D / K338N, E1K / M6L / A87T / T121P / I362L, D31N / D35Y / T177I / P207S / R266M, K24E / Q60H / N126D / T132I / T340I, D31N / D35Y // P207S / R266M / K33 T132I / P207S / R266M, Y114N / T177I / T181L / E182I / K183P, E1Q / S79F / Q252L / I362M / K369N, K24E / Q60H / N73D / D90V / K120R / D361V, D31N / D35Y20, D31N / D35Y D31K / D35Y / M130V / P207S / R266M / A371P, D31N / D35Y / F66Y / M130V / P207S / Q257E / N286T, D31N / D35Y / M130V / E182V / P207S / R266M / A371P, D20 / P3 R266M / A371P, D31N / D35Y / Q60H / G81E / M130V / P207S / R266M / A371P, K24E / D31N / D35Y / T121I / M130V / L154V / P207S / R266M / A371P, Q60K / F66Y Q252H / Q257E / A371P, Q60K / F66Y / N73P / N74P / Q75T / Q257E / I362L / P364Q / A371P, H (- 4) Q / D31N / D35Y / E53V / M130V / A148T / P207S / R266M / Q3, A / F66Y / N74P / T76G / Y114N / M130V / Y155F / T340I / I362F / A371P / D375 N, D31N / D35Y / F66Y / N73P / N74P // V83I / D90V / Y114N / N126D / N142D / Q252H / T340 / , D31N / D35Y / Q60K / F66Y / N73P / P80L // V83I // Y117N / N126D / Y155F / Q252H / Q257E / T340I / A371P, and D31N / D35Y / Q60K / F66Y / N74P / T76G // V83I / D90V / Y155F / Q252L / G321 / 340 / I362L / A371P.
    [12]
    12. Method according to claim 10, characterized in that the phytase produced comprises the modifications: A / B / K129P, A / B / K129Q, A / B / K129I, A / B / K129R, A / B / P207GA / B / N203L / P207G, A / B / K129R / N203L, 5 A / B / K129R / N203L / P207G, A / B / K24R, A / B / K24R / K129P, A / B / K24R / K129R / N203L / P207G, A / B / K24R / K129R / N203L, A / B / D, A / D / F66L / R94V / ∑a / E182K / K183Q / T185G / K186L / T188S / P190T / P207G / S2 13G / T217A / K336V , A / D / F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V, A / D / L64F / F66L / R94V / K116A / E119S / T188S / P190T / T217A, A / D / F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V / Q252W, A / D / F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V / Q252Y, A / D / F252Y, A / D / F252Y, A / D / F252Y R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V / Q252F, A / D / R94V / K116A / E119S / T188S / P190T / T217A, A / B / D / F66L / R94V / ∑a / T188 / P190T / P207G / S213G / T217A / K336V, A / B / D / F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V / Q252Y, A / B / D / R94V / K116A / E119S / T118 / P190T / T217A, A / D / R94V / K116A / E118T / E119S / K120S / N121R / T188S / P190T / S213G / T217 A / K336V, A / D / R94V / K116A / E118T / E119S / K120S / N12 / T12 P19 0T / S213G / T217 A, A / D / R94V / K116A / E118T / E119S / K120S / T188S / P190T / T217A, A / D / ∑a / E182K / K183Q / T185G / K186L / S213G / T217A / K336V, D F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V, A / D / F66L / R94V / ∑a / E182K / K183Q / T185G / K186L / T188S / P190T / P207G / S2 K2 , A / D / F66L / R94V / ∑a / T188S / P190T / P207G / S213G / T217A / K336V, D / F66L / R94V / ∑a / K179N / E182K / K183Q / T185G / K186L / T188S / P190T / P20 S213G / T217A / K336V,
    D / F66L / R94V / ∑a / E182N / K183Q / T185G / K186L / T188S / P190T / P207G / S213 G / T217A / K336V, D / ∑b / E182R / K183Q / T185G / K186L / S213G / T217, / F66L / ∑b / E182R / K183Q / T185G / K186L / T188S / P190T / S213G / T217A / K33 5 6R, D / F66L / ∑b / E182R / K183Q / T185G / K186L / T188S / P190T / E196 7A / K336R, D / ∑b / E182R / K183Q / T185G / K186L / S213G / T217A / K336R / K393G / R397Q / K400A, D / ∑b / E182K / K183Q / T185G / K186L / S213G / T217 / ∑c / K183Q / T185G / K186L / S213G / T217A, D / F66L / ∑b / E182R / K183Q / T185G / K186L / T188S / P190T / N203L / S213G, D / F66L / R94V / ∑d / E182K / / K186L / T188S / P190T / P207G / S213 G / T217A / K336V, A / D / R94V / K116A / E119S / T188S / P190T / T217A, A / B / D / F66L / R94V / ∑a / T188S / P190T / P20 / S213G / T217A / K336V, D / K116A / E119S / T217A, D / E1 * / E2 * / R94V / K116A / E119S / T188S / P190T / T217A, where A means G52C / A99C, B means N31C / T177C, C means W46C / Q91C, D means K141C / V199C, E means N31C / E176C, F means G59C / F100C, and G means S162C / S247C; and where ∑a means the replacement of the TQADTSSR fragment with the YQKDEEKN fragment at positions 114-121, ∑b means the replacement of the TQADTSSP fragment with the YQKDEEKN fragment, ∑c means the replacement of the TQADTSSN fragment with the YQKDEEKN fragment, ∑d means the replacement of the TQADTSS fragment with the YQKDEEKN fragment.
    [13]
    Method according to any one of claims 1 to 12, characterized in that the mature phytase part of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, or SEQ ID NO: 9 is used as a parent / backbone to produce a variant of phytase.
    [14]
    14. Phytase variant, characterized by the fact that it is produced by any of the methods as defined in any of claims 1 to 13, with a better thermostability, a better temperature profile, a better pH profile, better specific activity, better performance in animal feed and / or that incorporates a change in a potential protease cleavage site.
    [15]
    15. Isolated nucleic acid sequence, characterized in that it comprises a nucleic acid sequence encoding phytase as defined in claim 14.
    [16]
    16. Nucleic acid construct, characterized in that it comprises the nucleic acid sequence as defined in claim 15, operably linked to one or more control sequences that direct the production of phytase in a suitable expression host.
    [17]
    17. Recombinant expression vector, characterized by the fact that it comprises the nucleic acid construct as defined in claim 16.
    [18]
    18. Recombinant host cell, characterized by the fact that it comprises the nucleic acid construct as defined in claim 16, and / or the expression vector as defined in claim
    17.
    [19]
    19. Method for producing phytase as defined in claim 14, characterized in that it comprises: (a) culturing the host cell as defined in claim 18 to produce a supernatant comprising phytase; and (b) recovering the phytase.
    [20]
    20. Composition, characterized by the fact that it comprises at least one phytase as defined in claim 14, and (a) at least one fat-soluble vitamin;
    (b) at least one water-soluble vitamin; and / or (c) at least one trace mineral.
    [21]
    21. Composition according to claim 20, characterized in that it additionally comprises at least one enzyme selected from the following groups of enzymes: amylase, phytase, phosphatase, xylanase, galactanase, alpha-galactosidase, protease, phospholipase and / or beta -glucanase.
    [22]
    22. Method for improving the nutritional value of an animal feed, characterized by the fact that phytase as defined in claim 14 or the composition as defined in any of claims 20-21 is added to the feed.
    [23]
    23. Use of phytase as defined in claim 14 or the composition as defined in any of claims 20-21, characterized by the fact that it is in animal feed; in the preparation of animal feed; to improve the nutritional value of animal feed; to reduce phytase levels in animal manure; for the treatment of vegetable proteins; or to release phosphorus from a phytase substrate.
    [24]
    24. Method for producing a fermentation product, characterized in that it comprises (a) fermenting, using a fermenting microorganism, a material containing carbohydrate in the presence of a phytase as defined in claim 14, and (b) producing the fermentation product or fermentation by-product from material containing fermented carbohydrate.
    [25]
    25. Method according to claim 24, characterized by the fact that the fermentation product is ethanol, beer, wine, or dry distillation grains (DDG).
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    Rejab et al.2015|Possible contribution of TED6 and TED7, secondary cell wall-related membrane proteins, to evolution of tracheary element in angiosperm lineage
    Sitohy et al.2021|Protecting potato plants against PVX and PVY viral infections by the application of native and chemically modified legume proteins
    CN107532180A|2018-01-02|For obtaining the plasmid and method of virion
    RU2012102036A|2013-07-27|PROTEINS CONNECTING TO THE BIRD COLONY RECEPTOR 1 BIRD
    BR112019010476A2|2019-09-10|recombinant nucleic acid molecule, vector, host cell, transgenic plant, transgenic seed, recombinant polypeptide, composition, method for controlling a pest population, for killing pests, for producing a polypeptide, plant or plant cell, method for protecting a plant against a pest, to increase yield on a plant, use and primary product
    同族专利:
    公开号 | 公开日
    DK2553090T3|2019-05-13|
    ES2723050T3|2019-08-21|
    WO2011117396A3|2011-11-24|
    US20150159149A1|2015-06-11|
    BR112012023840B1|2021-06-22|
    AU2011231562B2|2015-10-22|
    PL2553090T3|2019-08-30|
    EP2553090B1|2019-02-27|
    US9611469B2|2017-04-04|
    US20130195829A1|2013-08-01|
    CA2794095A1|2011-09-29|
    AU2011231562A1|2012-09-27|
    CN102906254A|2013-01-30|
    EP2553090A2|2013-02-06|
    CA2794095C|2021-02-16|
    CA3105079A1|2011-09-29|
    WO2011117396A2|2011-09-29|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    WO2004085638A1|2003-03-25|2004-10-07|Republic Of National Fisheries Research And Development Institute|Phytase produced from citrobacter braakii|
    AU2005291727B2|2004-10-04|2012-02-02|Novozymes A/S|Polypeptides having phytase activity and polynucleotides encoding same|
    AR050895A1|2004-10-04|2006-11-29|Novozymes As|POLYPEPTIDES THAT HAVE FITASA ACTIVITY AND POLYUCLEOTIDES THAT CODE THEM|
    EP1797178B1|2004-10-04|2012-09-12|DuPont Nutrition Biosciences ApS|Citrobacter freundii phytase and homologues|
    GB0422052D0|2004-10-04|2004-11-03|Dansico As|Enzymes|
    ES2531434T3|2006-04-04|2015-03-16|Novozymes A/S|Phytase variants|
    US8540984B2|2006-08-03|2013-09-24|Cornell Research Foundation, Inc.|Phytases with improved thermal stability|
    EP2109356A2|2007-01-30|2009-10-21|Novozymes A/S|Polypeptides having phytase activty and polynucleotides encoding same|
    WO2008116878A1|2007-03-26|2008-10-02|Novozymes A/S|Hafnia phytase|
    AU2008352003B2|2007-12-03|2014-05-08|Syngenta Participations Ag|Engineering enzymatically susceptible phytases|
    EP2650364B1|2008-09-26|2015-05-20|Novozymes A/S|Hafnia phytase variants|
    FR2947563B1|2009-07-01|2015-08-21|Biomethodes|IMPROVED VARIANTS OF A PHYTASE|
    US8334124B1|2009-09-23|2012-12-18|The United States Of America As Represented By The Secretary Of Agriculture|Modified Aspergillus niger phytase|
    DK2491120T3|2009-10-22|2016-03-21|Basf Se|Synthetic phytasevarianter|
    CA2794113A1|2010-03-26|2011-09-29|Novozymes A/S|Thermostable phytase variants|
    CN102906255A|2010-03-26|2013-01-30|诺维信公司|Thermostable phytase variants|
    CA2825365A1|2011-02-18|2012-08-23|Dupont Nutrition Biosciences Aps|Feed additive composition|
    CN103502443B|2011-04-21|2016-05-04|巴斯夫欧洲公司|Synthetic phytase variants|MX2009003032A|2006-09-21|2009-11-18|Verenium Corp|Phytases, nucleic acids encoding them and methods for making and using them.|
    GB201102865D0|2011-02-18|2011-04-06|Danisco|Feed additive composition|
    GB201102857D0|2011-02-18|2011-04-06|Danisco|Feed additive composition|
    CA2825365A1|2011-02-18|2012-08-23|Dupont Nutrition Biosciences Aps|Feed additive composition|
    CN103649754B|2011-07-07|2016-11-02|杜邦营养生物科学有限公司|Algoscopy|
    GB201213801D0|2012-08-03|2012-09-12|Dupont Nutrition Biosci Aps|Feed additive composition|
    GB201308828D0|2013-03-12|2013-07-03|Verenium Corp|Phytase|
    GB201308843D0|2013-03-14|2013-07-03|Verenium Corp|Phytase formulation|
    EP2799531A1|2013-05-03|2014-11-05|Clariant ProdukteGmbH|Use of phosphatases for the enzymatic degumming of triglycerides|
    AR095173A1|2013-07-25|2015-09-30|Basf Enzymes Llc|FITASA|
    CN104450643B|2014-12-19|2020-07-28|青岛蔚蓝生物集团有限公司|Phytase mutant and application thereof|
    TWI591180B|2016-02-18|2017-07-11|基酵生物科技股份有限公司|Phytase having improved thermostability|
    TWI615471B|2016-02-18|2018-02-21|基酵生物科技股份有限公司|Phytase having improved thermostability|
    TWI607088B|2016-02-18|2017-12-01|基酵生物科技股份有限公司|Phytase having improved thermostability|
    TWI616532B|2016-02-18|2018-03-01|基酵生物科技股份有限公司|Phytase having improved thermostability|
    CN110129298B|2016-06-02|2021-08-03|天津科技大学|Phospholipase D mutant and application thereof|
    CN111867388A|2018-02-08|2020-10-30|丹尼斯科美国公司|Heat-resistant wax matrix particles for enzyme encapsulation|
    KR20210005665A|2018-04-26|2021-01-14|다니스코 유에스 인크.|Method for increasing the stability of phytase in solid composition and granule composition comprising phosphate and phytase|
    EA202092569A1|2018-05-30|2021-07-08|Наньцзин Бестзайм Био-Энжиниринг Ко., Лтд.|MODIFIED FITASES|
    AU2020310162A1|2019-07-09|2022-02-03|Dupont Nutrition Biosciences Aps|Fat coated particulate enzyme compositions|
    WO2021034660A1|2019-08-16|2021-02-25|Dupont Nutrition Biosciences Aps|Compositions for gut health comprising combinations of lactobacillus strains|
    WO2021046073A1|2019-09-05|2021-03-11|Dupont Nutrition Biosciences Aps|Feed composition|
    WO2021080864A2|2019-10-21|2021-04-29|Dupont Nutrition Biosciences Aps|Compositions for gut health|
    WO2021158927A1|2020-02-07|2021-08-12|Dupont Nutrition Biosciences Aps|Feed compositions for animal health|
    WO2022034211A1|2020-08-13|2022-02-17|Novozymes A/S|Phytase variants and polynucleotides encoding same|
    法律状态:
    2020-08-25| B06U| Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]|
    2020-12-22| B06A| Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]|
    2021-05-04| B09A| Decision: intention to grant [chapter 9.1 patent gazette]|
    2021-06-22| B16A| Patent or certificate of addition of invention granted|Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/03/2011, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, , QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO. |
    优先权:
    申请号 | 申请日 | 专利标题
    EP10158027.2|2010-03-26|
    EP10158027|2010-03-26|
    PCT/EP2011/054639|WO2011117396A2|2010-03-26|2011-03-25|Thermostable phytase variants|
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